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Elisa has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries This guide explains what an elisa (short for enzyme linked immunosorbent assay) is, the procedures involved, types of elisa, detection options and results In the most simple form of an elisa, antigens from the sample to be tested are attached to a surface.

Enzyme-linked immunosorbent assay (ELISA)

Elisa is a laboratory technique that detects certain antibodies, antigens and other substances in your blood, pee or other bodily fluid Learn about elisas (enzyme linked immunosorbent assays) and the various types of elisa including direct, indirect, sandwich and competitive elisas. Laboratory scientists use this technique for several medical tests — from diagnosing infections to confirming pregnancy.

Learn about the different methods for performing an elisa assay for protein quantitation, including assay design strategies and reagents.

It is a method of quantifying an antigen immobilized on a solid surface.